Generator
CqsA
Part:BBa_K356001
Designed by: C David Owen, James Taylor Group: iGEM10_St_Andrews (2010-09-01)
CAI-1 Generator
Vibrio cholerae CqsA with strong constituitive promotor, ribosome binding site and terminator. Generates CAI-1, a V. cholerae specific autoinducer which is detected by CqsS.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 71
Illegal suffix found in sequence at 1457 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 71
Illegal NheI site found at 27
Illegal NheI site found at 50
Illegal NheI site found at 99
Illegal NheI site found at 122
Illegal SpeI site found at 1458
Illegal PstI site found at 1472
Illegal NotI site found at 77
Illegal NotI site found at 1465 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 71
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 71
Illegal suffix found in sequence at 1458 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 71
Illegal XbaI site found at 86
Illegal SpeI site found at 1458
Illegal PstI site found at 1472 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds
protein |